Phenylalanine (Phe) Colorimetric Assay Kit
Katalog-Nummer E-BC-K846-M-96T
Size : 96T
Marke : Elabscience
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Detection Principle | Phenylalanine (Phe), chemically known as 2-amino-3-phenylpropionic acid, is an essential amino acid involved in a variety of biosynthetic processes. In the body, most Phe is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthefies important neurotransmitters and hormones, and participates in glucose metabolism and fat metabolism. Phenylalanine is catalyzed by enzyme , and the chromogenic agent has an absorption peak at 450 nm. The Phe content of the sample can be calculated by measuring the OD value of the chromogenic substance at 450 nm. |
| Sample Type | Serum,Plasma,Urine,Animal and plant tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (430-470 nm, optimum wavelength: 450 nm) |
| Research Area | Liver And Renal Function , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4.25 μmol/L |
| Detection Range | 4.25-500.00 μmol/L |
| Precision | inter-assay CV: 2.4-4.0% | intra-assay CV: 0.9-1.6% |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 2-4 |
| 10% Mouse stomach tissue homogenate | 2-3 |
| 10% Peach flesh tissue homogenate | 1 |
| Rat plasma | 2-3 |
| Human serum | 2-4 |
| Rabbit serum | 2-3 |
| 1×10^6 Jurka cells | 1 |
| 1×10^6 Mouse spleen cells | 1 |
| Jurka cell culture supernatant | 2-4 |
| Mouse spleen cell culture supernatant | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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