Polyclonal Antibody to a-internexin/NF66

Figure-1: Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to α-internexin,(34-1055), dilution 1:2,000, in green, and chicken pAb to GFAP,(34-1046), dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The α-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.