Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Polymyxin B nonapeptide [86408-36-8]

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Katalog-Nummer HY-106783-50mg

Size : 50mg

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Telefonnummer : +1 850 650 7790

Marke : MedChemExpress

Contact local distributor :

Telefonnummer : +1 850 650 7790

Polymyxin B nonapeptide is a cyclic peptide obtained from Polymyxin B by proteolytic removal of its terminal amino acyl residue. Polymyxin B nonapeptide is less toxic, lacks bactericidal activity, and retains its ability to render gram-negative bacteria susceptible to several antibiotics by permeabilizing their outer membranes.

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Polymyxin B nonapeptide Chemische Struktur

CAS. Nr. : 86408-36-8

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of Polymyxin B nonapeptide:

Polymyxin B nonapeptide TFA In-stock

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Alle Isoformen anzeigen

Beschreibung

In Vitro

Polymyxin B nonapeptide (2-32 μg/mL, 24 h) has a synergistic effect with Azithromycin (HY-17506)^[1].
Polymyxin B nonapeptide (10 μg/mL) inhibits  lipopolysaccharide (HY-D1056)-induced neutrophilic activation in a concentration-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	E. coli strains
Concentration:	2, 4, 8, 16, 32 μg/mL
Incubation Time:	24 h
Result:	Combinated with AZT caused a significant reduction the bacterial growth after 6 h and complete killing after 24 h.

In Vivo

Polymyxin B nonapeptide (1.5, 3.0 mg/kg intravenously for 29 days) does not exhibit neuromuscular blocking, neurotoxic, or nephrotoxic effects in rats as observed with polymyxin B sulfate^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

963.13

Formel

C₄₃H₇₄N₁₄O₁₁

CAS. Nr.

86408-36-8

Appearance

Solid

Color

White to off-white

Sequence

Thr-{Dab}-{Dab}-{Dab}-d-Phe-Leu-{Dab}-{Dab}-Thr (Lactam: Dab3-Thr9)

Sequence Shortening

T-{Dab}-{Dab}-{Dab}-d-FL-{Dab}-{Dab}-T (Lactam: Dab3-Thr9)

SMILES

O=C(N[C@H](C(N[C@@](C(N[C@H](C(N[C@]([C@H](O)C)(22)C(NCC[C@@](NC([C@H](CCN)NC([C@@H](N)[C@H](O)C)=O)=O)(22)C(N[C@@H](CCN)C1=O)=O)=O)=O)CCN)=O)(22)CCN)=O)CC(C)C)[C@H](N1)CC2=CC=CC=C2

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 100 mg/mL (103.83 mM)

DMSO : 16.67 mg/mL (17.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0383 mL	5.1914 mL	10.3828 mL
5 mM	0.2077 mL	1.0383 mL	2.0766 mL
10 mM	0.1038 mL	0.5191 mL	1.0383 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (1.73 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Reinheit & Dokumentation

Purity: 99.82%

Select Batch:

Data Sheet (285 KB) SDS (252 KB)

COA (263 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Al-Marzooq F, et al. Discerning the role of polymyxin B nonapeptide in restoring the antibacterial activity of azithromycin against antibiotic-resistant Escherichia coli. Front Microbiol. 2022 Sep 21;13:998671. [Content Brief]

[2]. Danner RL,et al. Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide. Antimicrob Agents Chemother. 1989 Sep;33(9):1428-34. [Content Brief]

[3]. Ofek I, et al. Antibacterial synergism of polymyxin B nonapeptide and hydrophobic antibiotics in experimental gram-negative infections in mice. Antimicrob Agents Chemother. 1994 Feb;38(2):374-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.0383 mL	5.1914 mL	10.3828 mL	25.9570 mL
	5 mM	0.2077 mL	1.0383 mL	2.0766 mL	5.1914 mL
	10 mM	0.1038 mL	0.5191 mL	1.0383 mL	2.5957 mL
	15 mM	0.0692 mL	0.3461 mL	0.6922 mL	1.7305 mL
H₂O	20 mM	0.0519 mL	0.2596 mL	0.5191 mL	1.2979 mL
	25 mM	0.0415 mL	0.2077 mL	0.4153 mL	1.0383 mL
	30 mM	0.0346 mL	0.1730 mL	0.3461 mL	0.8652 mL
	40 mM	0.0260 mL	0.1298 mL	0.2596 mL	0.6489 mL
	50 mM	0.0208 mL	0.1038 mL	0.2077 mL	0.5191 mL
	60 mM	0.0173 mL	0.0865 mL	0.1730 mL	0.4326 mL
	80 mM	0.0130 mL	0.0649 mL	0.1298 mL	0.3245 mL
	100 mM	0.0104 mL	0.0519 mL	0.1038 mL	0.2596 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Polymyxin B nonapeptide Related Classifications

Anti-infection
Bacterial Antibiotic

Polymyxin B nonapeptide [86408-36-8]

Katalog-Nummer HY-106783-50mg

Contact local distributor :

Marke : MedChemExpress

Contact local distributor :

Other Forms of Polymyxin B nonapeptide:

Alle Antibiotic Isoform-spezifische Produkte anzeigen:

Polymyxin B nonapeptide Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Polymyxin B nonapeptide Related Classifications

Keywords: