These antibody-free human primary cells were initiated from normal human coronary arterial tissue without the use of positive selection of antibodies.
Primary Human Coronary Artery Smooth Muscle Cells (ACBRI 443)
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- PRODUCT INFO
- CITATIONS
- TESTS
- DOCUMENTATION
Increase Biological Relevance with Human Primary Cells
Our antibody-free primary cells can offer a more biologically relevant cell culture tool for scientists to enhance their research insights. These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705).
- Isolated from normal, healthy donor tissue
- Available at Passage 3 (<12 cumulative population doublings)
- Each vial contains approximately 1 x 106 cells
- Each vial will initiate a Passage 4 cell culture in a 75 cm2 flask
- Available in reserved lots to enhance consistency in your research program
Each vial of cells is shipped to customers with 1mL of Bac-Off® and .25mL of CultureBoost™.
A Selection of Citations for ACBRI 443 from Scientific Journals
Discover additional research on Google Scholar that utilizes Cell Systems primary cells.
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"Distinct Transcriptional Regulation of Human Large Conductance Voltage- and Calcium-activated K+ Channel Gene (hSlo1) by Activated Estrogen Receptor α and c-Src Tyrosine Kinase" Danesh et al. J Biological Chemistry, 2011.
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"Alzheimer Disease-Associated Peptide, Amyloid beta 40, Inhibits Vascular Regeneration With Induction of Endothelial Autophagy" Hayashi et al. Arteriosclerosis, Thrombosis, and Vascular Biology, 2009.
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"Oxidized Phospholipid Inhibition of Toll-like Receptor (TLR) Signaling Is Restricted to TLR2 and TLR4" Erridge et al. J Biological Chemistry, 2008.
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"High-precision fluorescence assay for sphingomyelinase activity of isolated enzymes and cell lysates" Loidl et al. Journal of Lipid Research, 2002.
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"Inhibition of vascular cell growth by X-ray irradiation: comparison with gamma radiation and mechanism of action" Scott et al. J Radiation Oncology, 2001.
Standard Tests
| TESTS | RESULTS |
| HIV Serologic Test (donor level HIV AB EIA) | Negative |
| HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) | Negative |
| Hepatitis B (HBV) and Hepatitis C (HCV) PCR Test (at frozen cell level) | Negative |
Retail Production (P3)Tests
| TESTS | RESULTS |
| Bacterial Sterility (culture method) by independent lab | Pass |
| Fungal Sterility (culture method) by independent lab | Pass |
| Mycoplasma Sterility (culture method) by independent lab | Pass |
Cell Markers and Functional Tests
| TESTS | RESULTS |
| Desmin Intermediate Filaments | > 95% positive by immunofluorescence |
| Cytoplasmic alpha-Actinin Microfilaments | > 95% positive by immunofluorescence |
| Cytoplasmic uptake of Di-I-Ac-LDL | < 01% by immunofluorescence |
