The Prostaglandin E2 (PGE2) BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of FSH in mouse serum, plasma, tissue homogenates and other biological fluids.||Detection Range:|31.25-2000pg/ml||Sensitivity:|18.75pg/ml||Precision:|Intra-Assay CV: <8%|Inter-Assay CV: <10%||Assay Principle:|The microtiter plate provided in this kit has been pre-coated with PGE2. PGE2 in the standard or sample competes with a fixed amount of plate-coated PGE2 for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of PGE2 in the samples is determined by an inverse correlation between PGE2 in the sample and the assay signal intensity.||Kit Components:|*358066A: Microtiter Strips, 1x48 wells or 1x96 wells |*358066B: Standard, 1x1 vial or 2x1 vial|358066C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml|358066D: Antibody-Biotin (Concentrated), 1x30ul or 1x60ul|358066E: Antibody Dilution Buffer, 1x5ml or 1x10ml|358066F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul|358066G: SABC Dilution Buffer, 1x5ml or 1x10ml|358066H: TMB Substrate, 1x5ml or 1x10ml|358066J: Stop Solution, 1x5ml or 1x10ml|358066K: Wash Buffer, 25X, 1x15ml or 1x30ml||Storage and Stability:|Store unopened *358066A at 4ºC; store at -20ºC once opened. Store unopened *358066B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Summary:|1. Wash plate 2 times before adding standards and samples to wells!|2. Add 50ul standard or sample to each well.|3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37°C|4. Aspirate and wash 3 times.|5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C|6. Aspirate and wash 5 times.|7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C|8. Add 50ul Stop Solution. Read at 450nm immediately.|9. Calculate results