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Datasheet SDS Protein A Resin is an affinity chromatography resin with high binding capacity for IgG. Protein A Resin is suitable for purification of monoclonal antibodies, polyclonal antibodies and immunology complexes, such as IP and Co-IP. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. Specifications:
Research Articles on Protein A Resin
- Resin: Cross-linked 4% agarose
- Ligand: Recombinant Protein A
- Shape: Sphere
- Pore Size: 90 µm (45-165)
- Support Density: 6 mg Protein A/ml wet gel
- Binding Capacity: 40-50 mg h-IgG/ml wet gel
- Maximum Flow Rate (25 °C): 300 cm/h
- Recommended Flow Rate: < 150 cm/h
- Highest Resisistance of Atmospheric Pressure: 0.3 MPa
- pH Stability: 3-10
| Source | IgG Subtype | Affinity for Protein A | Affinity for Protein G |
| Human | IgG1 | ++++ | ++++ |
| Human | IgG2 | ++++ | ++++ |
| Human | IgG3 | - | ++++ |
| Human | IgG4 | ++++ | ++++ |
| Mouse | IgG1 | + | ++++ |
| Mouse | IgG2a | ++++ | ++++ |
| Mouse | IgG2b | +++ | +++ |
| Mouse | IgG3 | ++ | +++ |
| Rabbit | IgG | ++++ | +++ |
| Goat | IgG | - | ++ |
| Horse | IgG | ++ | ++++ |
| Dog | IgG | ++ | + |
| Cow | IgG | ++ | ++++ |
| Pig | IgG | +++ | +++ |
| Monkey | IgG | ++++ | ++++ |
| Target | Protein A Resin |
| Storage | Store at 2-8 °C (with 20% ethanol) for up to 2 years. |
| Buffer | Note: Buffers are not included with this product. Equilibration Buffer: 20 mM PBS, 150 mM KCl, pH 7.0 Elution Buffer: 20 mM citric acid, pH 3.0-4.0; or 100 mM glycine, pH 3.0; or 20 mM sodium acetate, pH 3.0-4.0 Neutralisation Buffer: 1 M Tris-HCl, pH 9.0 |
| Availability | Shipped within 10-20 working days. |
| Note | This product is for research use only. |
| Directions for use | Preparing the Protein A purification column:
To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading. Loading samples and washing:Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube Elute:Elute antibodies with elution buffer. Collect the elution containing the target immunoglobulin and immediately neutralise to pH > 7.0 with neutralisation buffer. The elution conditions are closely related with binding strength and stability of antibody. When necessary, optimise the elution buffer. Regeneration of Protein A resin:
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