Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-312aa
- UniProt No.
- P61889
- NCBI Accession No.
- NP_417703
- Alternative names
- malate dehydrogenase, ECK3225, JW3205
Product Specification
- Molecular Weight
- 34.9 kDa (336aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 1000unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.