Recombinant Human IFN alpha2a（E.coli）

Biological Activity

Genus: Human
Expression system: E.coli
Purity: >95% by reduced SDS-PAGE
Endotoxin: less than 0.1 ng/µg (1 EU/µg) by LAL test
Apparent molecular weight: 16 KDa, reduced conditions
Storage and stability: Lyophilized proteins should be stored at <-20°C, although stable at room temperature for up to 3 weeks. Reconstituted protein solutions can be stored at 4-7°C for 2-7 days. Recombinant sample aliquots are stable for 3 months at <-20°C.

Bioactivity: Human interferon Alpha 2a (IFN-Alpha 2a), produced by leukocytes, is a member of the interferon family. IFN-alpha is primarily involved in the innate immune response against a broad range of viral infections. there are three acidic stable forms of IFN-alpha 2 (a,b,c) that signal through IFNAR2. IFN-alpha 2a shares 99.4% and 98.8% sequence identity with IFN-alpha 2b and 2c, respectively. IFN-alpha contains four highly conserved cysteine residues that form two disulfide bonds, one of which is required for biological activity.

Chemical Information

Solubility (25°C)	It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Paulo V S Dias, et al. J Chromatogr B Analyt Technol Biomed Life Sci. Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography

[2] Y Mohammed, et al. Biochemistry (Mosc). Expression of human interferon-α8 synthetic gene under P(BAD) promoter

[3] J Meng, et al. Cytotherapy. High-yield expression, purification and characterization of tumor-targeted IFN-alpha2a

[4] Zhen Yan, et al. Appl Biochem Biotechnol. Expression, refolding, and characterization of GFE peptide-fused human interferon-alpha2a in Escherichia coli

[5] E Baron, et al. Crit Rev Biotechnol. From cloning to a commercial realization: human alpha interferon