SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448]
Katalog-Nummer GTX635686-25ul
Size : 25ul
Marke : GeneTex
Contact local distributor :
Telefonnummer : +1 850 650 7790
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Host | Rabbit |
|---|---|
Clonality | Monoclonal |
Clone Name | HL448 |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) |
Reactivity | SARS Coronavirus, SARS Coronavirus 2 |
| Package | 100 μl, 25 μl |
Datasheet File APPLICATION
Application Note
| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:10000 |
| ICC/IF | 1:100-1:1000 |
| IHC-P | 1:5000-1:10000 |
| ELISA | Assay dependent |
| Lateral Flow | Assay dependent |
| Sandwich ELISA | Assay dependent |
| IHC-P (cell pellet) | Assay dependent |
| Note : IHC-PRecommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes and background Eraser (BGE) blocking step. Lateral Flow Capture : GTX635688, Detection : GTX635686 Please notice that the detection antibodies need to be conjugated to Gold to function when paired with capture antibodies. Please contact us for custom Gold-conjugated antibody. Sandwich ELISA Capture : GTX635686 , Detection: GTX635678 / GTX635688. or Capture : GTX635685 / GTX635689 / GTX635679 , Detection: GTX635686-01. Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody. | |
Product Note
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DATA IMAGES
| | GTX635686 ELISA Image Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL. |
| | GTX635686 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.011 nM |
| | GTX635686 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 IHC-P (cell pellet) ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
| | GTX635686 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. |
| | GTX635686 IHC-P ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.The IHC-P was performed by HISTOWIZ. |
| | GTX635686 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 WB ImageSARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635686 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635686 ICC/IF ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920). |
| | GTX635686 WB ImageNon-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 WB ImageSARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635686 Lateral Flow ImageDetection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control. Capture: S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])Detection:S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])Samples (40 ng) : 1. Lysis buffer2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro) |
| | GTX635686 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) (1000-15.625 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody. |
| | GTX635686 IHC-P ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).The IHC-P was performed by HISTOWIZ. |
| | GTX635686 ELISA ImageSandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody. |
Application Reference
REVIEW
| SDS | |
|---|---|
| PBS.pdf | |