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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to SARS-CoV N Protein antigen.

Sandwich ELISA
Application

Sandwich ELISA

A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) as a detection antibody. SARS-CoV-2 N Protein was serially diluted 1:2 starting at 25 ng/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) and SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) detected SARS-CoV-2 N Protein antigen at very high sensitivity as low as 100 pg/mL.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against SARS-CoV-2 N.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    A synthetic peptide corresponding to C-terminus of SARS-CoV-2 N.

    Reactivity

    SARS-CoV-2

    Specificity

    Highly-specific to SARS-CoV-2 nucleoprotein; does not cross-react with nucleoprotein from SARS-CoV or other coronaviruses.

    Form

    Liquid

    Conjugation

    Biotin

    Preparation Method

    84C4a is derived from a Fab phage display library made from a rabbit immunized with synthetic peptide specific to the C-terminus of SARS-CoV-2 nucleocapsid protein. Libraries were selected on N (SARS-CoV-2) Recombinant Protein (Cat # P6687) and Fab sup were screened on SARS-CoV-2 NP and SARS-CoV (2003) NP by ELISA. Positive clones were cloned into a bi-cistronic IgG vector and produced in HEK293 cells. The antibodies were purified from a Protein A column.

    Purification

    Protein A purification

    Concentration

    0.5 mg/mL

    Isotype

    IgG

    Recommend Usage

    ELISA
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.02% sodium azide)

    Storage Instruction

    Store at 4°C.

    Note

    75G5a (Cat # RAB01053), 84C4a (Cat # RAB01055), 84D7 (Cat # RAB01057), 85C1 (Cat # RAB01059), 85C10 (Cat # RAB01061), 85B4 (Cat # RAB01063), and 85E9 (Cat # RAB01065) can be directly coated to the ELISA wells as a capture antibody and the other antibody (e.g. biotinylated) [75G5a (Cat # RAB01054), 84C4a (Cat # RAB01056), 84D7 (Cat # RAB01058), 85C1 (Cat # RAB01060), 85C10 (Cat # RAB01062), 85B4 (Cat # RAB01064), and 85E9 (Cat # RAB01066)] can be used as a detecting antibody with StreptAvidin-HRP. For highest sensitivity, we recommend pairing unconjugated clone 75G5a (Cat # RAB01053), immobilized on the ELISA plate to capture COVID-19 NP antigen, with SARS-CoV-2 N recombinant monoclonal antibody, clone 85C10 (Biotin) (Cat # RAB01062) for detection using high-sensitivity streptavidin-HRP (Pierce #21130).
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Microtiter wells were coated with N (SARS-CoV-2) Recombinant Protein (Cat # P6687) at 1 ug/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to SARS-CoV N Protein antigen.

    Sandwich ELISA

    A sandwich ELISA was performed using SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) as a capture antibody and SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) as a detection antibody. SARS-CoV-2 N Protein was serially diluted 1:2 starting at 25 ng/mL. SARS-CoV-2 N recombinant monoclonal antibody, clone 75G5a (Cat # RAB01053) and SARS-CoV-2 N recombinant monoclonal antibody, clone 84C4a (Biotin) (Cat # RAB01056) detected SARS-CoV-2 N Protein antigen at very high sensitivity as low as 100 pg/mL.