Secretory Component (Rhodamine)

Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. ||Applications: |Suitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Rhodamine/IgG protein molar ratio (F/P): |~1.5||Excitation: |554nm||Emission: |573nm||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Rhodamine conjugates are light sensitive.