Tissue Fixation Solution / 4% Paraformaldehyde (with DEPC)
Katalog-Nummer E-IR-R114-100
Size : 100mL
Marke : Elabscience
Product Introduction | 4% Paraformaldehyde tissue fixation solution is widely used in the detection of tissue, tissue slice, cell and other biological sample fixation solutions such as immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FCM). This product is prepared in PBS solution and can be directly used for tissue or cell fixation without dilution. If a lower concentration of paraformaldehyde is needed, PBS can be used as dilution buffer. 4% Paraformaldehyde tissue fixation solution has strong penetrability and fixation, which can make the tissue harden and it is good for slicing. It will cause less tissue shrinkage, less damage and mild, which can well preserve the inherent substance and maintain the antigenicity and fine structure of the tissue. In addition, 4% Paraformaldehyde can be used to fix and preserve fat and lipid substances. This product has good fixation effect and wide applications. It is suitable for the fixation of various common cells or tissues. It has good fixation effect on skin, muscle, viscera, etc. It mainly acts on protein, but can’t fix uric acid and sugar, etc. It is recommended that 1 ml of fixed solution is needed for each sample fixation. |
Applications | IHC, IF, FCM |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Cautions | 1. If the product is stored for a long time, the aldehyde group may be oxidized to acid, which will reduce the pH of the solution, thus will affect the subsequent dyeing. 2. Different cell or tissue samples need different fixation time. The fixation time should be adjusted according to the type of cell or tissue and the size of tissue block. 3. Although the effect of 4% Paraformaldehyde tissue fixation solution is mild, it may harden the tissue. If the fixation time is too long, the tissue will become brittle and fragile. Therefore, the fixation time should not exceed 24 hours. 4. Polyoxymethylene can exist in the fixed cell or tissue samples for a long time. There will still be residues after washing with appropriate detergent or water for several hours. Therefore, the subsequent experimental results are easy to be affected by aldehyde group, and the residual polyoxymethylene must be washed away as much as possible. 5. 4% Paraformaldehyde tissue fixation solution fix the cells or tissue samples need to be repaired before immunostaining or other subsequent operations. 6. This product is harmful to human body. Please be careful during operation and pay attention to effective protection to avoid direct contact with human body or inhalation. 7. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 8. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
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