Application
| WB, ICC |
|---|---|
| Primary Accession | Q12931 |
| Other Accession | NP_057376.2 |
| Host | Mouse |
| Isotype | IgG2a Kappa |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human Trap1 Monoclonal IgG2a Kappa |
| Target/Specificity | Detects ~75kDa. |
| Other Names | Heat shock protein 75 Antibody, Heat shock protein 75 kDa Antibody, Heat shock protein 75 kDa mitochondrial Antibody, HSP 75 Antibody, HSP 90L Antibody, HSP75 Antibody, HSP90L Antibody, mitochondrial Antibody, TNF receptor associated protein 1 Antibody, TNFR associated protein 1 Antibody, TNFR-associated protein 1 Antibody, TRAP 1 Antibody, TRAP-1 Antibody, Trap1 Antibody, TRAP1_HUMAN Antibody, Tumor necrosis factor receptor associated protein Antibody, Tumor necrosis factor type 1 receptor associated protein Antibody, Tumor necrosis factor type 1 receptor-associated protein Antibody |
| Clone Names | Trap1-6 |
| Immunogen | Purified recombinant TRAP1 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-193 was sufficient for detection of Trap-1/HSP75 in 20 µg of Hela Cell lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Mitochondrion |
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Provided below are standard protocols that you may find useful for product applications.
Background
The 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
References
1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312.
2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-46.
3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.