Tsg101 Polyclonal Antibody
Katalog-Nummer bs-1365R-20ul
Size : 20ul
Marke : Bioss
Contact local distributor :
Telefonnummer : +1 850 650 7790
Tsg101 Polyclonal Antibody
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain lysates, L2 rat heart lysates probed with Anti-Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 42kD
Lane 1: MCF-7 cell lysates probed with Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1365R |
| Product Name | Tsg101 Polyclonal Antibody |
| Applications | WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Reactivity | Human, Mouse, Rat, Fish |
| Predicted Reactivity | Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tsg101 |
| Immunogen Range | 4-120/390 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7251 |
| Swiss Prot | Q99816 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | TSG10; VPS23; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101; TSG101 |
| Background | Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
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