We manufacture a number of standards intended for use with either image-based cell viability analyzers (e.g. trypan blue, Vi-CELL®) or flow cytometers (DAPI, propidium iodide, LIVE/DEAD® stains, etc) that are important to bioprocessing and research programs.
ViaCheck™ Viability and Concentration Standards
Our ViaCheck™ standards mimic the characteristics of live and dead cells in the trypan blue dye exclusion method and may be used to support validation and QC of image-based cell viability analyzers (e.g. Vi-CELL®) used in biomanufacturing programs. ViaCheck™ Viability Controls are supplied at ~1.0 x 106 beads / mL and are available in a range of live/dead ratios. ViaCheck™ Concentration Controls are supplied at the noted concentrations.
PDS 706, ViaCheck™ Viability Controls
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, Optimization of Vi-CELL® Settings for Calibration checks using ViaCheck™ Controls
TSD 0711, ViaCheck™ for Cell Viability Analyzers: Best Practices
Video: ViaCheck™ Protocol
TSD 0712, Repeatability of Coulter Z2 and Vi-CELL Counts using ViaCheck™ 0.50e+6 Concentration Controls
SDS ST352, (ViaCheck™ Viability)
SDS ST353, (ViaCheck™ Concentration)
Catalog Number | Description | Volume (mL) |
---|---|---|
VC10B | ViaCheck™ 0% Viability Control | 20 |
VC25B | ViaCheck™ 25% Viability Control | 20 |
VC20B | ViaCheck™ 50% Viability Control | 20 |
VC30B | ViaCheck™ 75% Viability Control | 20 |
VC40B | ViaCheck™ 90% Viability Control | 20 |
VC50B | ViaCheck™ 100% Viability Control | 20 |
VC50N | ViaCheck™ Concentration Control (0.5 × 106) | 20 |
VC60N | ViaCheck™ Concentration Control (1 × 106) | 20 |
VC70N | ViaCheck™ Concentration Control (4 × 106) | 20 |
VC80N | ViaCheck™ Concentration Control (8 × 106) | 20 |
ViaCheck™ Viability and Concentration Standards - SingleShots™
ViaCheck™ SingleShots™, affordable single-use QC. Mix, dispense & run for confirmation of live/dead ratios and counts. ViaCheck™ SingleShots™ minimize error and maximize efficiency by circumventing potential human error from repeated bottle resuspension & pipetting steps.
PDS 706, ViaCheck™ Viability Controls
PDS 711, ViaCheck™ Concentration Controls
TSD 0706, Handling & Pipetting Concentration Standards
Appl.Note 0708, Optimization of Vi-CELL® Settings for Calibration checks using ViaCheck™ Controls
TSD 0711, ViaCheck™ for Cell Viability Analyzers: Best Practices
Video: ViaCheck™ Protocol
TSD 0712, Repeatability of Coulter Z2 and Vi-CELL Counts using ViaCheck™ 0.50e+6 Concentration Controls
SDS ST352, (ViaCheck™ Viability)
SDS ST353, (ViaCheck™ Concentration)
Catalog Number | Description | Units |
---|---|---|
VC10BSS | ViaCheck™ 0% Viability Control SingleShots™ | 25 or 75 |
VC25BSS | ViaCheck™ 25% Viability Control SingleShots™ | 25 or 75 |
VC20BSS | ViaCheck™ 50% Viability Control SingleShots™ | 25 or 75 |
VC30BSS | ViaCheck™ 75% Viability Control SingleShots™ | 25 or 75 |
VC40BSS | ViaCheck™ 90% Viability Control SingleShots™ | 25 or 75 |
VC50BSS | ViaCheck™ 100% Viability Control SingleShots™ | 25 or 75 |
VC50NSS | ViaCheck™ Concentration Control (0.5 × 106) SingleShots™ | 25 or 75 |
VC60NSS | ViaCheck™ Concentration Control (1 × 106) SingleShots™ | 25 or 75 |
VC70NSS | ViaCheck™ % Concentration Control (4 × 106) SingleShots™ | 25 or 75 |
VC80NSS | ViaCheck™ % Concentration Control (8 × 106) SingleShots™ | 25 or 75 |
References
Kaminski RW, Wu M, Turbyfill KR, Clarkson K, Tai B, Bourgeois AL, Van De Verg LL, Walker RI, Oaks EV. (2014) Development and preclinical evaluation of a trivalent, formalin-inactivated Shigella whole-cell vaccine. Clin Vaccine Immunol; 21(3):366-382.
Cadena-Herrera D, Esparza-De Lara JE, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E. (2015) Validation of three viable-cell counting methods: manual, semi-automated, and automated. Biotechnol Reports; 7:9-16.
Lew C, Gomez J, Rhyner M (2012) Instrument-to-instrument Variability in the Vi-CELL Automated Viability Analyzer. Beckman Coulter Life Sciences
Chen A, Leith M, Tu R, Tahim G, Sudra A, Bhargava S. (2017) Effects of diluents on cell culture viability measured by automated cell counter. PLoS One; 12(3):e0173375.
Pagliuca F W, Millman JR, Gürtler M, Segel M, Van Dervort A, Ryu JH, ... & Melton DA. (2014). Generation of functional human pancreatic β cells in vitro. Cell, 159(2), 428-439.
Fluorescence Reference Standards - FLOW
We offer microspheres labeled with traditional dyes/stains that may be used as standards to support flow cytometric assays in cell cycle analysis or cell viability determinations. Beads may be used for routine instrument set-up and QC, or as test-specific standards. See also our full range of Fluorescence Reference Standards .
PDS 890, Fluorescence Reference Standards
Brochure: Cell Cycle Analysis
SDS ST331, (Fluorescence Reference Standards)
SDS ST334, (Fluorescence Reference Standards)
Catalog Number | Description |
---|---|
897 | Acridine Orange |
906 | DAPI |
891 | Fluorescein (FITC) |
894 | Hoechst 33342 |
892 | Propidium Iodide |
Viability Dye Compensation Standard - Flow
Viability Dye Compensation Standards are suitable for labeling with LIVE / DEAD® stains or other amine-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatibility of a specific dye or stain. Supplied at ~1e+7 beads/mL.
PDS 853, Viability Dye Compensation Standard
SDS ST340, (VIability Dye Compensation Standard)
Catalog Number | Description |
---|---|
450 | Viability Dye Compensation Standard, 4µm |
451 | Viability Dye Compensation Standard, 8µm |
References
Estes, Myka L., et al. "Identification of endothelial cells and progenitor cell subsets in human peripheral blood." Current protocols in cytometry (2010): 9-33.