Application
| WB, IF, FC, IHC-P-Leica, E |
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Primary Accession | P08670 |
Other Accession | NP_003371.2 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Clone/Animal Names | RB41034 |
Calculated MW | 53652 Da |
Gene ID | 7431 |
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Other Names | Vimentin, VIM |
Target/Specificity | This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein. |
Dilution | IF~~1:50 WB~~1:2000 IHC-P-Leica~~1:500 FC~~1:50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VIM |
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Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152} |
Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |