K2224-100 assays | Xanthine Oxidase Activity Colorimetric/Fluorometric

Background

Xanthine oxidase (XO) is a kind of xanthine oxidoreductase that generates reactive oxygen species. XO is present in the jejunum and liver in healthy individuals. However, XO is released into circulation in various liver disorders. Measurement of serum XO level is a sensitive indicator of acute liver damage such as jaundice.

The Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for accurate detection of XO activity in various samples based on colorimetric and fluorometric method. In the assay, XO oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with the Probe to yield fluorescence (at Ex/Em = 535/587 nm) and color (at λ = 570 nm). The fluorescence intensity or color generated is proportional to XO content. The kit can detect 1-100 mU xanthine oxidase in 100 µL reaction volume.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. less than 40 minutes; Sensitive assays for measuring xanthine oxidase activity in various biological samples.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

XO Assay Buffer, OxiRed™ Probe (in DMSO), XO Enzyme Mix, XO Substrate Mix, XO Positive Control, H₂O₂ Standard (0.88 M)

Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

Katalog-Nummer K2224-100 assays

Contact local distributor :

Marke : APExBIO Technology