10X ImmunoStain wash buffer
Katalog-Nummer GTX73344
Size : 1liter
Marke : GeneTex
Application | IHC |
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Package | 1 liter |
View product citations for antibody GTX73344 on CiteAb
PRODUCT
Summary
10X ImmunoStain wash buffer (pH 7.5)
APPLICATION
Application Note
No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques.
PROPERTIES
Storage
Store at room temperature.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining. In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. Immuno Wash Buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining.
Application Reference
Elkabets M et al. Methods Mol Biol 2018; 1806 : 155-176 Methods to Study the Role of Progranulin in the Tumor Microenvironment.
REVIEW
Application Reference
Elkabets M et al. Methods Mol Biol 2018; 1806:155-176 Methods to Study the Role of Progranulin in the Tumor Microenvironment.