Product Description  

5 min TA/Blunt-Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase, a vector containing the suicide gene ccdB and blunting factors. The second-generation Topoisomerase is matched with the optimal buffer, which has higher activity and further improves the cloning efficiency. Efficient ligation can be achieved by reacting at room temperature for 5 min. The vector contains the suicide gene ccdB. When the insert is successfully connected to the vector, the expression of the ccdB gene will be disrupted, and the host cell can grow normally, otherwise the host cell will die, thus realizing the "Zero" background. The blunting factor makes this product compatible with both TA cloning and blunt-end cloning.

 Features  

• Compatible: Suitable for sticky ends, blunt ends, TA , linker or adapter ligation

• Fast: Cloning of either sticky- or blunt-end DNA in only 5 minutes

• Efficient: High efficiency cloning and positive rate >95%

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.