Acid Phosphatase (ACP) Activity Assay Kit (PNPP Method)

Katalog-Nummer E-BC-K010-M-500

Size : 500Assays

Marke : Elabscience

Contact local distributor :


Telefonnummer : +1 850 650 7790

Detection Principle Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.
Synonyms ACP
Sample Type Serum,Plasma,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (400-415 nm, optimum wavelength: 405 nm)
Research Area Liver And Renal Function
Other Reagents Required PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.2 U/L
Detection Range 0.2-50 U/L
Precision inter-assay CV: 4.5% | intra-assay CV: 2.8%
Assay Time 30 min
Cat.No. Product Name Sizes
E-BC-K091-M Alkaline Phosphatase (ALP) Activity Assay Kit 500Assays , 96T , 48T
E-BC-K094-M Acid Phosphatase (ACP) Activity Assay Kit 500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Epipremnum aureum tissue homogenate 5-10
Mouse plasma 5-10
Rat plasma 5-10
Human urine 1
Human plasma 5-10
10% Rat spleen tissue homogenate 20-30
10% Rat liver tissue homogenate 20-30
10% Rat kidney tissue homogenate 20-30

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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