ADH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommended Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/20,000).
Reactivities	Bakers Yeast
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Alcohol dehydrogenase isolated and purified from Baker's Yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Alcohol Dehydrogenase.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized IgG fraction. Label: Conjugation Procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Molar radio: ~5.5
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation	Biotin
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Shelf life: one year from despatch. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P00330
Background	ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.
Synonyms	Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
Reference Data