Mouse Monoclonal

Application / Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 31 kDa

Isotype IgG1

Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.

 

References
Lee, J.H. et al. (2008) J Biol. Chem. May 19 epub.
Zukerberg, L. R. (2000) Neuron 26:633.
Poon, R.Y.C. et al. (1997) J Biol. Chem. 272( 9):5703.

Western blot analysis of human HeLa cells. The blot was probed with anti-Cdk5 (CM2361) antibody at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000.(lane 3).



Clones DC17+DC34 were generated from a recombinant full-length human Cdk5 sequence.

*For more information, see UniProt Accession Q00535
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 31 kDa* band corresponding to Cdk5 on SDS-PAGE immunoblots of human SYF and HeLa cells, and in mouse brain tissue. The antibody does not detect other Cdk family members, such as Cdk1 and Cdk2.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: