Application
| WB, ICC |
|---|---|
| Primary Accession | Q86TL0 |
| Other Accession | NP_001268433.1 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human ATG4D Polyclonal |
| Target/Specificity | Detects ~52kDa. |
| Other Names | APG4 autophagy 4 homolog D Antibody, APG4 D Antibody, ATG4 autophagy related 4 homolog D Antibody, AUTL4 Antibody, Cysteine protease ATG4D Antibody, MGC31226 Antibody |
| Immunogen | Synthetic peptide from the C-terminal of human ATG4D |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-634 was sufficient for detection of ATG4D on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@clinisciences.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
References
1. "Entrez Gene: Autophagy related 4D, cysteine peptidase".
2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.