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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263 (FITC) (Cat # MAB9988 ).
Lane 1: lysate of human PBL.
Lane 2: lysate of canine PBL.
Lane 3: lysate of porcine PBL.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against CD44.

    Immunogen

    COS-7 cells (African Green Monkey)

    Host

    Mouse

    Reactivity

    Dog, Human, Monkey, Pig

    Specificity

    The antibody reacts with extracellular (N-terminal) domain of standard CD44, a 80-95 kDa transmembrane glycoprotein (hyaladherin family)present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.

    Form

    Liquid

    Conjugation

    FITC

    Isotype

    IgG1

    Recommend Usage

    Flow cytometry (20 ul reagent / 100 ul of whole blood or 106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide, 0.2% (w/v) BSA)

    Storage Instruction

    Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze.

  • Applications

    Western Blot (Cell lysate)

    Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263 (FITC) (Cat # MAB9988 ).
    Lane 1: lysate of human PBL.
    Lane 2: lysate of canine PBL.
    Lane 3: lysate of porcine PBL.

    Flow Cytometry