CD46 monoclonal antibody, clone MEM-258 (PE)
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Flow Cytometry
Surface staining of human peripheral blood cells with CD46 monoclonal antibody, clone MEM-258 (PE) (Cat # MAB4442).
- Specifications
Product Description
Mouse monoclonal antibody raised against native CD46.
Immunogen
Native purified CD46 from human T cell line HPB-ALL.
Host
Mouse
Theoretical MW (kDa)
56-66
Reactivity
Bovine, Human
Specificity
This antibody recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 KDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast; it is negative on erythrocytes.
Form
Liquid
Conjugation
PE
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot
Immunoprecipitation
Flow Cytometry
Surface staining of human peripheral blood cells with CD46 monoclonal antibody, clone MEM-258 (PE) (Cat # MAB4442). - Gene Info — CD46
Entrez GeneID
4179Gene Name
CD46
Gene Alias
MCP, MGC26544, MIC10, TLX, TRA2.10
Gene Description
CD46 molecule, complement regulatory protein
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. This gene is found in a cluster on chromosome 1q32 with other genes encoding structural components of the complement system. At least fourteen different transcript variants encoding fourteen different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD46 antigen, complement regulatory protein|OTTHUMP00000034577|OTTHUMP00000034622|OTTHUMP00000034623|OTTHUMP00000034624|OTTHUMP00000034625|OTTHUMP00000034626|OTTHUMP00000034706|antigen identified by monoclonal antibody TRA-2-10|complement membrane cofacto
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation.
Oliaro J, Pasam A, Waterhouse NJ, Browne KA, Ludford-Menting MJ, Trapani JA, Russell SM.
PNAS 2006 Nov; 103(49):18685.
- Emerging roles and new functions of CD46.
Liszewski MK, Kemper C, Price JD, Atkinson JP.
Springer Seminars in Immunopathology 2005 Nov; 27(3):345.
- CD46 is a cellular receptor for group B adenoviruses.
Gaggar A, Shayakhmetov DM, Lieber A.
Nature Medicine 2003 Oct; 9(11):1408.
- Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation.