CYP7A1 Human qPCR Primer Pair (NM_000780)
CAT#: HP200721
qSTAR qPCR primer pairs against Homo sapiens gene CYP7A1
SensiMix SYBR Master Mix
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Specifications
Product Data | |
Gene ID | 1581 |
Forward Sequence | CAAGCAAACACCATTCCAGCGAC |
Reverse Sequence | ATAGGATTGCCTTCCAAGCTGAC |
Accession No | NM_000780, NM_000780.1, NM_000780.2, NM_000780.3, BC101777, BC101777.1, BC112184, NM_000780.4 |
UniProt ID | P22680 |
Synonyms | CP7A; CYP7; CYPVII |
Component | 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM. |
Quality Control | The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec. |
Storage | Store at -20°C. |
Stability | The primer mix is stable for one year from date of shipping. |
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