Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.04]
Specifications
Product Data | |
Clone Name | Ks18.04 |
Applications | FC, IF, IHC |
Recommended Dilution | Immunofluorescence. Cytological material. Flow Cytometry: 1/5-1/10. Immunohistochemistry on Frozen Sections: 1/10. Immunohistochemistry on Paraffin Sections: 1/10. Incubation Time: 1 h at 37°C. |
Reactivities | Bovine, Canine, Hamster, Human, Mouse, Porcine, Rat, Sheep, Trout |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human keratin K18 from a HeLa cytoskeletal preparation |
Specificity | Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tested Tumors Specifically Detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with HeLa and MCF-7 (cell lines tested so far). |
Formulation | Label: FITC State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide Label: Fluorescein |
Purification | Affinity Chromatography on Protein A |
Conjugation | FITC |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | keratin 18 |
Database Link | |
Background | Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
Synonyms | Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18 |
Reference Data |
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