ASR1855 | F(ab')2 Anti-Human IgG F(c) Pre-Adsorbed Secondary Antibody

Western Blot of Goat anti-F(ab')2 Human IgG F(c) Antibody (Pre-Adsorbed) Lane 1: Human IgG F(c) Load: 100 ng per lane Primary antibody: F(ab')2 Human IgG F(c) Antibody (Pre-Adsorbed) at 1:1000 o/n at 4°C Secondary antibody: DyLight™ 800 Donkey anti-goat at 1:20,000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 28 kDa, 28 kDa

Product Information
Description	F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Antibody Min X Bv Hs Ms & Rt Serum Proteins
Host	Goat
Conjugate	Unconjugated
Target Species	Human
Clonality	Polyclonal
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, E, IC
Application Note	ELISA 1:1,500-1:7,000 Western Blot 1:500-1:5,000 Immunochemistry 1:1,000-1:5,000
Physical State	Liquid (sterile filtered)
Host Isotype	IgG F(ab')2
Target Isotype	IgG F(c)
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen	Human IgG F(c) fragment
Stabilizer	None
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse, Mouse and Rat Serum Proteins.
Storage Condition	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

F(ab')2 Anti-Human IgG F(c) Pre-Adsorbed Secondary Antibody