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> F(ab')2 Anti-Rat IgG (H&L) (Texas Red™ Conjugated) Pre-Adsorbed Secondary Antibody

F(ab')2 Anti-Rat IgG (H&L) (Texas Red™ Conjugated) Pre-Adsorbed Secondary Antibody

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Katalog-Nummer ASR3178

Size : 500uL

Marke : Abcepta

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Telefonnummer : +1 850 650 7790

 
  • WB - F(ab')2 Anti-Rat IgG (H&L) (Texas Red™ Conjugated) Pre-Adsorbed Secondary Antibody ASR3178
    Western Blot of Texas Red™ conjugated Donkey F(ab’)2 Anti-Rat IgG (Pre-Adsorbed) secondary antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rat IgG. Other band(s): Rat IgG splice varients and isoforms.
Product Information
Description F(ab')2 Anti-RAT IgG [H&L] (DONKEY) Antibody Texas Red™ Conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins
Host Donkey
Conjugate Texas Red®
FP Value 5.8 moles Texas Red® per mole of IgG F(ab')2
Target Species Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF
Application Note IF Microscopy 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG whole molecule
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.