Goat anti-CDX2
Katalog-Nummer EB07190
Size : 100ug
Marke : Everest Biotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Code | Name | Applications | Availability | Size | Tested species | Grade |
|---|---|---|---|---|---|---|
| EB07190 | Goat Anti-CDX2 Antibody | Pep-ELISA, WB, IHC | 100µg specific antibody in 200µl | Human |
EB07190 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07190 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Target Protein
Principal Names: CDX2, caudal type homeo box transcription factor 2, HGNC:1806, CDX-3, CDX3Official Symbol: CDX2
Accession Number(s): NP_001256.1
Human GeneID(s): 1045
Non-Human GeneID(s): 12591 (mouse), 66019 (rat)
Important Comments: No cross-reactivity expected with CDX1 and 4
Immunogen
Peptide with sequence EQLSPGGQRRNLCE, from the internal region of the protein sequence according to NP_001256.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:4000.Western blot: Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Colon. Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol
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