HEL D1.3 Human IgM Isotype Control, In Vivo Grade Recombinant Human

The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The HEL D1.3-based recombinant isotype control and mutants (natural and engineered isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. As an ideal recombinant mouse IgG1 isotype control, the recombinant HEL D1.3 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions. The mouse IgG1 kappa isotype control and mutants can be used in ELISA, WB, the cell-based assay or animal model as the antibody negative control.||The in vivo grade recombinant HEL D1.3 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.||Recombinant anti-HEL isotype control antibodies and Fc silenced mutants from clone HEL D1.3 good for in vitro and in vivo studies. Low or no specific binding to human, rat, and mouse samples tested.||Applications: |Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and in vivo animal model research. Other applications not tested.||Recommended Dilution:|Immunohistochemistry: frozen/paraffin|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.