Human HDAC3 (Histone Deacetylase 3) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	HDAC3
Gene ID:	8841
Uniprot ID:	HDAC3_HUMAN
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Tissue Specificity	Widely expressed.
Post Translational Modifications	Sumoylated in vitro. Deubiquitinated on 'Lys-63'-linked ubiquitin chains by USP38.leading to a decreased level of histone acetylation.
Function	Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress. Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner. During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver. Also functions as deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14 and RARA. Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively. Catalyzes decrotonylation of MAPRE1/EB1.
Protein Name	Histone Deacetylase 3 Hd3 Protein Deacetylase Hdac3 Protein Deacylase Hdac3 Rpd3-2 Smap45
Database Links	Reactome: R-HSA-1368071 Reactome: R-HSA-193670 Reactome: R-HSA-1989781 Reactome: R-HSA-2122947 Reactome: R-HSA-2151201 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-3214815 Reactome: R-HSA-350054 Reactome: R-HSA-381340 Reactome: R-HSA-390471 Reactome: R-HSA-400206 Reactome: R-HSA-400253 Reactome: R-HSA-5617472 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9022537 Reactome: R-HSA-9022692 Reactome: R-HSA-9029569 Reactome: R-HSA-9609690 Reactome: R-HSA-9701898 Reactome: R-HSA-9707564 Reactome: R-HSA-9707616
Cellular Localisation	Nucleus Cytoplasm Cytosol Colocalizes With Xbp1 And Akt1 In The Cytoplasm Predominantly Expressed In The Nucleus In The Presence Of Ccar2
Alternative ELISA Names	Histone Deacetylase 3 ELISA kit Hd3 ELISA kit Protein Deacetylase Hdac3 ELISA kit Protein Deacylase Hdac3 ELISA kit Rpd3-2 ELISA kit Smap45 ELISA kit HDAC3 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance