Macrophage Colony Stimulating Factor, Recombinant, Human, Animal Free (M-CSF, CSF-1, MGI-IM)

Katalog-Nummer 208678-10ug

Size : 10ug

Marke : US Biological

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208678 Macrophage Colony Stimulating Factor, Recombinant, Human, Animal Free (M-CSF, CSF-1, MGI-IM)

Clone Type
Polyclonal
Grade
Highly Purified
Shipping Temp
Blue Ice
Storage Temp
-20°C

Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, macrophages and endothelial cells. This colonly stimulating factor is involved in both the development and function of monocytes/macropahges. Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of 158aa residues.||Source:|Recombinant protein corresponding to human M-CSF, expressed in E. coli.||Biological Activity:|Determined by its ability to stimulate the proliferation of murine M-NFS-60 cells. The expected ED50 is <1.0ng/ml, corresponding to a specific activity of >1x10e6units/mg.||Endotoxin:|<0.1ng/ug of protein (<1EU/ug)||AA Sequence:|MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD|QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ|LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN|VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS||Protein Content:|Verified by UV Spectroscopy and/or SDS-PAGE gel.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: E. coli|Purity: ~98% (SDS-PAGE gel, HPLC)|Concentration: ~0.1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder in Sodium Phosphate, pH 8.0, 75mM sodium chloride. Reconstitute with ddH2O.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder in sodium phosphate, pH 8.0, 75mM sodium chloride. Reconstitute with ddH2O.
Purity
~98% (SDS-PAGE gel, HPLC)