NIR-H2O2
Katalog-Nummer T19038-100mg
Size : 100mg
Marke : TargetMol
NIR-H2O2
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NIR-H2O2
Catalog No. T19038
NIR-H2O2 is a cell-permeable near-infrared (NIR) fluorescent turn-on sensor that exhibits a significant turn-on NIR fluorescence signal in response to H2O2 upon excitation in the NIR region.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. Pack Size | |
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100 mg |
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
Description | NIR-H2O2 is a cell-permeable near-infrared (NIR) fluorescent turn-on sensor that exhibits a significant turn-on NIR fluorescence signal in response to H2O2 upon excitation in the NIR region. |
In vitro | NIR-H2O2 is highly selective to H2O2 over other typical ROS and bio-relevant species. HeLa cells incubated with NIR-H2O2 (5 μM, 30 min, 37 °C) exhibit minimal fluorescence, but show strong fluorescence upon H2O2 treatment. Similarly, RAW264.7 macrophages loaded with NIR-H2O2 (1 μM) display almost no fluorescence, but when co-incubated with PMA (3.0 μg/mL), show significant red emission. NIR-H2O2 effectively enables fluorescent imaging of endogenous H2O2 in living RAW264.7 macrophages. |
In vivo | In an acute inflammation model induced by lipopolysaccharide (LPS), in vivo hydrogen peroxide (H2O2) production was generated by activated macrophages and neutrophils. Mice treated with both LPS and near-infrared-responsive H2O2 (NIR-H2O2) displayed significantly higher fluorescence readouts than those untreated or treated solely with NIR-H2O2. Specifically, fluorescence intensity in mice administrated with LPS and NIR-H2O2 was approximately 10- and 20-fold greater compared to mice treated with saline and the sensor, and mice treated with saline alone, respectively. |
Molecular Weight | 565.89 |
Formula | C34H33BClNO4 |
Cas No. |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSO
main solution, add 300 μLPEG300
mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O
mix well and clarify
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
NIR H2O2NIRH2O2NIR-H-2O2NIR-H2O2
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