Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
> NODAL Protein Lysate

NODAL Protein Lysate

Drucken Drucken
Melden Sie sich an

Katalog-Nummer OCOA02393

Size : 20ug

Marke : Aviva Systems Biology

Contact local distributor :


Telefonnummer : +1 850 650 7790

    Datasheets/ManualsPrintable datasheet for NODAL Protein Lysate (OCOA02393)
    Product Info
    Predicted Species ReactivityHomo sapiens
    Product FormatLyophilized
    HostHEK293T
    Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
    ::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
    Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
    TagC-MYC/DDK
    Gene SymbolNODAL
    Gene Full Namenodal growth differentiation factor
    Alias SymbolsHTX5;nodal homolog.
    NCBI Gene Id4838
    Protein NameNM_018055, NP_060525, Q96S42
    Description of TargetHomo sapiens nodal growth differentiation factor (NODAL), transcript variant 1, mRNA, Nodal homolog, nodal homolog isoform 1 preproprotein [Homo sapiens]
    Uniprot IDhttps://www.uniprot.org/uniprot/Q96S42
    Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_060525
    Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_018055
    Molecular Weight39.58 kDa