Product Details
Applications: ICC, WB, IHC
Type: Rabbit IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken
Entrez:
UniProt:
Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons.
Section of rat spinal cord stained with NF-H, phosphylated (green) and monoclonal antibody to UCHL1 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons.