Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Phalloidin, CF®555

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Katalog-Nummer 00040-T

Size : 50U

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Biotium

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Phalloidin Conjugates

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

Product Attributes

Probe cellular localization	Cytoskeleton, F-Actin
For live or fixed cells	For fixed cells
Assay type/options	Tissue staining
Detection method/readout	Fluorescence microscopy
Cell permeability	Membrane impermeant
Fixation options	Fix before staining (formaldehyde), Permeabilize before staining
Toxin	Phalloidin
Colors	Blue, Green, Orange, Red, Far-red, Near-infrared

Technology

Cellular Stains

CF® Dye & Other Bioconjugates

CF® Dyes

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Product Description

A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized cells.

Choice of 17 bright & photostable CF® Dyes, biotin, or other common fluorophores
Dye options for super-resolution and 2-photon imaging
Excellent signal to background with negligible non-specific staining

Fluorescently-labeled phalloidins bind to F-actin with nanomolar affinity. Labeled phalloidins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants, animals and fungi. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin among different species.

Fluorescently-labeled phalloidin is typically used to stain fixed and permeabilized cells. Labeled phalloidins are not cell-permeant and have therefore not been used extensively with living cells, however they can be loaded into live cells via cationic liposomes.

Note: Phalloidin stock solutions are very stable. But binding of fluorescent phalloidins to cells is more labile than other probes, such as antibodies, and staining can become non-specific or lost over time. For best results, store phalloidin-stained samples in a suitable mounting medium at 4°C, protected from light. For certain phalloidin conjugates, especially CF®405M, CF®647, and CF®680, we recommend imaging immediately or shortly after staining. Staining with our other CF® Dye phalloidins usually is stable for up to a week when specimens are stored at 4°C, protected from light.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Super-Resolution Microscopy

CF®647 and CF®680 phalloidins are recommended mainly for STORM applications. Certain CF® Dyes are compatible with various super resolution imaging techniques. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.

Phalloidin Conjugates

Product	Conjugation	Ex/Em	Size	Catalog No.	Purchase
Biotin-XX Phalloidin	Biotin-XX	N/A	100 U	00028	Purchase 00028
CF®350 Phalloidin	CF®350	347/448 nm	50 U	00049-T	Purchase 00049-T
CF®350 Phalloidin	CF®350	347/448 nm	300 U	00049	Purchase 00049
CF®405M Phalloidin	CF®405M	408/452 nm	50 U	00034-T	Purchase 00034-T
CF®405M Phalloidin	CF®405M	408/452 nm	300 U	00034	Purchase 00034
CF®430 Phalloidin	CF®430	426/498 nm	50 U	00054-T	Purchase 00054-T
CF®430 Phalloidin	CF®430	426/498 nm	300 U	00054	Purchase 00054
CF®440 Phalloidin	CF®440	440/515 nm	50 U	00055-T	Purchase 00055-T
CF®440 Phalloidin	CF®440	440/515 nm	300 U	00055	Purchase 00055
CF®488A Phalloidin	CF®488A	490/515 nm	50 U	00042-T	Purchase 00042-T
CF®488A Phalloidin	CF®488A	490/515 nm	300 U	00042	Purchase 00042
CF®532 Phalloidin	CF®532	527/558 nm	50 U	00051-T	Purchase 00051-T
CF®532 Phalloidin	CF®532	527/558 nm	300 U	00051	Purchase 00051
CF®543 Phalloidin	CF®543	541/560 nm	50 U	00043-T	Purchase 00043-T
CF®543 Phalloidin	CF®543	541/560 nm	300 U	00043	Purchase 00043
CF®568 Phalloidin	CF®568	562/583 nm	50 U	00044-T	Purchase 00044-T
CF®568 Phalloidin	CF®568	562/583 nm	300 U	00044	Purchase 00044
CF®583R Phalloidin	CF®583R	586/609 nm	50 U	00064-T	Purchase 00064-T
CF®583R Phalloidin	CF®583R	586/609 nm	300 U	00064	Purchase 00064
CF®594 Phalloidin	CF®594	593/614 nm	50 U	00045-T	Purchase 00045-T
CF®594 Phalloidin	CF®594	593/614 nm	300 U	00045	Purchase 00045
CF®633 Phalloidin	CF®633	630/650 nm	50 U	00046-T	Purchase 00046-T
CF®633 Phalloidin	CF®633	630/650 nm	300 U	00046	Purchase 00046
CF®640R Phalloidin	CF®640R	642/662 nm	50 U	00050-T	Purchase 00050-T
CF®640R Phalloidin	CF®640R	642/662 nm	300 U	00050	Purchase 00050
CF®647 Phalloidin*	CF®647	650/665 nm	50 U	00041-T	Purchase 00041-T*
CF®647 Phalloidin*	CF®647	650/665 nm	300 U	00041	Purchase 00041*
CF®660C Phalloidin	CF®660C	667/685 nm	50 U	00052-T	Purchase 00052-T
CF®660C Phalloidin	CF®660C	667/685 nm	300 U	00052	Purchase 00052
CF®660R Phalloidin	CF®660R	663/682 nm	50 U	00047-T	Purchase 00047-T
CF®660R Phalloidin	CF®660R	663/682 nm	300 U	00047	Purchase 00047
CF®680 Phalloidin*	CF®680	681/698 nm	50 U	00053-T	Purchase 00053-T*
CF®680 Phalloidin*	CF®680	681/698 nm	300 U	00053	Purchase 00053*
CF®680R Phalloidin	CF®680R	680/701 nm	50 U	00048-T	Purchase 00048-T
CF®680R Phalloidin	CF®680R	680/701 nm	300 U	00048	Purchase 00048
Fluorescein Phalloidin	Fluorescein	496/516 nm	300 U	00030	Purchase 00030
Rhodamine Phalloidin	Rhodamine	540/565 nm	300 U	00027	Purchase 00027
Rhodamine 110 Phalloidin	Rhodamine 110	502/524 nm	300 U	00032	Purchase 00032
Sulforhodamine 101 (Texas Red®) Phalloidin	Texas Red®	591/608 nm	300 U	00033	Purchase 00033

*CF®647 and CF®680 phalloidins are recommended for STORM applications, but due to the instability of staining with these conjugates, we do not recommend using them for other microscopy applications. For other applications requiring far-red phalloidins, we recommend CF®633, CF®640R, or CF®680R phalloidins, which have more stable binding.

Note: CF®555 Phalloidin (00040, 00040-T) has been discontinued. We recommend CF®568 Phalloidin as a replacement for conventional microscopy, or CF®583R Phalloidin as a replacement for STORM.

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.