Rex1 (ZFP42) (NM_174900) Human Tagged ORF Clone

CAT#: RG223916

  • TrueORF®

ZFP42 (tGFP-tagged) - Human zinc finger protein 42 homolog (mouse) (ZFP42)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol Rex1
Synonyms REX-1; REX1; zfp-42; ZNF754
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG223916 representing NM_174900
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGCAACTGAAGAAACGGGCAAAGACAAGACACCAGAAAGGCCTGGGTGGAAGAGCCCCCAGTG
GGGCTAAGCCCAGGCAAGGCAAGTCAAGCCAAGACCTGCAGGCGGAAATAGAACCTGTCAGCGCGGTGTG
GGCCTTATGTGATGGCTATGTGTGCTATGAGCCTGGCCCTCAGGCTCTCGGAGGGGATGATTTCTCAGAC
TGTTACATAGAATGCGTCATAAGGGGTGAGTTTTCTCAACCCATCCTGGAAGGGGACTCACTTTTTGAGT
CCTTGGAATACCTAAAGAAAGGATCAGAACAACAGCTTTCTCAAAAGGTTTTCGAAGCAAGCTCCCTTGA
ATGTTCTTTGGAATACATGAAAAAAGGGGTAAAGAAAGAGCTTCCACAAAAGATAGTTGGAGAGAATTCG
CTTGAGTATTCTGAGTACATGACAGGCAAGAAGCTTCCGCCTGGAGGAATACCTGGCATTGACCTATCAG
ATCCTAAACAGCTCGCAGAATTTGCTAGAAAGAAGCCCCCCATAAATAAAGAATATGACAGTCTGAGCGC
AATCGCTTGTCCTCAGAGTGGATGCACTAGGAAGTTGAGGAATAGAGCTGCCCTGAGAAAGCATCTCCTC
ATTCATGGTCCCCGAGACCACGTCTGTGCGGAATGTGGGAAAGCGTTCGTTGAGAGCTCAAAACTAAAGA
GACATTTCCTGGTTCATACTGGAGAGAAGCCGTTTCGGTGCACTTTTGAAGGGTGCGGAAAGCGCTTCTC
TCTGGACTTTAATTTGCGTACGCACGTGCGCATCCACACGGGGGAGAAACGTTTCGTGTGTCCCTTTCAA
GGCTGCAACAGGAGGTTTATTCAGTCAAATAACCTGAAAGCCCACATCCTAACGCATGCAAATACGAACA
AGAATGAACAAGAGGGAAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG223916 representing NM_174900
Red=Cloning site Green=Tags(s)

MSQQLKKRAKTRHQKGLGGRAPSGAKPRQGKSSQDLQAEIEPVSAVWALCDGYVCYEPGPQALGGDDFSD
CYIECVIRGEFSQPILEGDSLFESLEYLKKGSEQQLSQKVFEASSLECSLEYMKKGVKKELPQKIVGENS
LEYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARKKPPINKEYDSLSAIACPQSGCTRKLRNRAALRKHLL
IHGPRDHVCAECGKAFVESSKLKRHFLVHTGEKPFRCTFEGCGKRFSLDFNLRTHVRIHTGEKRFVCPFQ
GCNRRFIQSNNLKAHILTHANTNKNEQEGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_174900
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_174900.2, NP_777560.1
RefSeq Size 2649 bp
RefSeq ORF 933 bp
Locus ID 132625
UniProt ID Q96MM3
Cytogenetics 4q35.2
Protein Families Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency
Gene Summary Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions