SK-MEL-5
Marke : CLS Cell Lines Service
SK-MEL-5 Cells
General information
Description | This is one of a very extensive series of melanoma lines which have been isolated by T. Takahashi and associates. The lines served as source of target cells for the detection of melanoma specific antibody in patients with this disease. |
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Organism | Human |
Tissue | Skin |
Disease | Melanoma |
Metastatic site | Axillary lymph node |
Synonyms | SK-Mel-5, SK MEL 5, SK.MEL.5, SK-MEL5, SKMel-5, SKMEL-5, SKMEL5, SKMel5, SKmel5, AA-Mel |
Characteristics
Age | 24 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Stellate |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-MEL-5 (Cytion catalog number 300157) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Isoenzymes | PGM1, 1-2, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0860 |
Tumorigenic | Yes, in nude mice, forms malignant melanoma |
Products | Melanin |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 TH01: 6,9 TPOX: 11 vWA: 14,18 D3S1358: 16,17 D21S11: 29 D18S51: 15,16 Penta E: 5,12 Penta D: 9,11 D8S1179: 12,15 FGA: 20.2,22 |
HLA alleles | A*: 02:01:01, 11:01:01 B*: 07:02:01, 40:01:02 C*: 03:04:01, 07:02:01 DRB1*: 04:01:01, 13:01:01 DQA1*: 01:03:01, 03:01:01 DQB1*: 03:02:01, 06:03:01 DPB1*: 03:01:01, 16:01:01 E: 01:01, 01:03 |