SK-MES-1
Marke : CLS Cell Lines Service
SK-MES-1 Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Squamous cell carcinoma |
Metastatic site | Pleural effusion |
Synonyms | SK MES 1, SKMES-1, SK-Mes-1, SK-MES1, SKMES1, SK-MES, SKMES |
Characteristics
Age | 65 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-MES-1 (Cytion catalog number 300339) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative |
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Isoenzymes | Me-2, 1-2, PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B, Phenotype Frequency Product: 0.0132 |
Karyotype | The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal x, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 12 D13S317: 11 D16S539: 13 D5S818: 11 D7S820: 8 TH01: 6,9.3 TPOX: 8 vWA: 14 D3S1358: 16 D21S11: 29,30 D18S51: 17 Penta E: 5,11 Penta D: 12,13 D8S1179: 13,14 FGA: 20,24 |
HLA alleles | A*: 03:01:01 B*: 07:02:01 C*: 07:02:01 DRB1*: 16:01:01 DQA1*: 01:02:02 DQB1*: 05:02:01 DPB1*: 04:01:01 E: 01:03:02 |