Superoxide Dismutase (SOD) BioAssay™ Kit (WST-1 Method)

Katalog-Nummer 405825-1Kit

Size : 1Kit

Marke : US Biological

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405825 Superoxide Dismutase (SOD) BioAssay™ Kit (WST-1 Method)

Clone Type
Polyclonal
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Superoxide dismutase plays a vital role in body balancing status of oxidation and antioxidation. This enzyme can remove superoxide anion free radical, protect cells from damage. The activity of SOD was measured by WST-1 method in this kit. Water-soluble tetrazolium, the sodium salt of 4-[3-(4iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), was used as a detector of superoxide radical generated by xanthine oxidase and hypoxanthine. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD. Therefore, the inhibition activity of SOD can be determined by a colorimetric method.||Sample Type:|Serum, plasma, cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cells, white blood cells, platelets, myocardial cells, tumor cells and a variety of plant and animal tissues and cells, subcellular level (mitochondria and microsome).||Intended Use:|This kit adopts the xanthine oxidase (hydroxylamine method) to measure SOD activity. The activity of SOD in Serum, plasma, cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cells, white blood cells, platelets, myocardial cells, tumor cells and a variety of plant and animal tissues and cells, subcellular level (mitochondria and microsome) can be tested by this kit. And the activity of SOD in microorganisms, medicine, food, beverage, cosmetics and other samples can be tested by this kit too.||Sensitivity: |0.5U/mL.||Precision: |Intra-Assay CV: <5.50%|Inter-Assay CV: <3.32%||Kit Components (see Protocol for final kit quantities):|Reagent 1, 15mL × 2vial:|Buffer Solution||Reagent 2, 0.15mL × 1vial:|Substrate Solution||Reagent 3, 0.3mL ×1vial:|Enzyme Solution||Reagent 4, 4mL × 1vial:|Enzyme Diluent||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Vachiranuvathin P, Jantaro S. Effect of alcohol stress on cell growth and its recovery of Synechocystis sp. PCC 6803[J].|Sample Type: Synechocystis sp. PCC 6803 wild type. 2. Siboto A, Sibiya N, Khathi A, et al. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers[J]. Journal of Diabetes Research, 2018, 2018.