TransStart® IV Reverse Transcriptase is an M-MLV reverse transcriptase with high thermostability expressed and purified from E. coli after genetic modification. It enables a board range reaction temperature (42°C-65°C) and good resistance to inhibitors, with ultra-high thermal stability, , and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of fast reaction speed, high sensitivity, high specificity, high yield (more full-length cDNA), and long half-life.

Features

• Wide range of applications: severely degraded RNA can be used as a template for reverse transcription.

• Agnostic to inhibitors remaining in RNA.

• Fast reaction speed: only 10 minutes for reverse transcription.

• Strong synthesis ability: synthesis length up to 20kb.

• High thermal stability: reaction temperature 42°C-65°C.

• High sensitivity: high detection rate for trace RNA.

Applications

• High-copy number and low-copy number gene detection.

• High GC or complex secondary structure RNA template.

• For difficult-to-handle RNA sample: degraded RNA template and RNA template containing RT enzyme inhibitor.

• cDNA library construction, primer extension, 3’ and 5’ RACE.

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)