Ubiquitin C-Terminal Hydrolase L1 (UCHL1) BioAssay™ ELISA Kit (Rat)

Katalog-Nummer 517742-96T

Size : 96Tests

Marke : US Biological

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517742 Ubiquitin C-Terminal Hydrolase L1 (UCHL1) BioAssay™ ELISA Kit (Rat)

Clone Type
Polyclonal
Swiss Prot
Q00981
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Sample Type:|Tissue homogenates, cell lysates, cell culture supernates and other biological fluids||Intended Use:|The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of UCHL1 in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.||Sensitivity:|0.25ng/ml||Range:|0.78-50ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.||Precision:|Intra-Assay: CV<10% |Inter-Assay: CV<12%||Test Principle:|The microplate provided in this kit has been pre-coated with an antibody specific to UCHL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to UCHL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UCHL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UCHL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|96-well strip plate, 1x Plate |Standard, 2x vial|Detection Reagent A, 1×120ul|Detection Reagent B, 1×120ul|TMB Substrate, 1×9ml|Wash Buffer (30X), 1×20ml|Standard Diluent, 1×20ml|Assay Diluent A, 1×12ml|Assay Diluent B, 1×12ml|Stop Solution, 1x6ml||Storage and Stability:|Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.