ViralBoost Reagent

Katalog-Nummer VB100

Size : 1mL

Marke : Alstem

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Telefonnummer : +1 850 650 7790

ViralBoost Reagent

Highlights:
  • Regulates packaging in transcriptional level
  • Works for both lenti-and retro-viral packaging
  • Increases viral particle production up to 10-fold
  • Achieve high titer at low cost
  • Novel cocktail of small molecules
  • Easy to use
Description:

ViralBoost Reagent (500X) is a novel cocktail of small molecules that is used for high titer virus production and is a powerful, broadly applicable reagent for effective virus packaging. It stably regulates the viral RNA packaging on the transcriptional level, which can greatly enhance production of either retro- or lenti-viral particles up to 10 fold. The easy-to-use protocol makes ViralBoost Reagent well-suited for various scales of virus packaging.

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Fig 1. Flow cytometry data from HEK 293T cells transduced by GFP retroviruses which were packaged in the absence and presence of the ViralBoost Reagent.
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Fig 2. HEK 293T cells transduced by GFP lentivirus which were packaged with and without ViralBoost
Specifications:
Product Name ViralBoost Reagent
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at 4 °C. This product is stable for 12 months when stored as directed.

Quality Control

Each lot is functionally tested in transfection assays using 293T cells.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

  1. I. Hollerer et al., Evidence for an Integrated Gene Repression Mechanism based on mRNA Isoform Toggling in Human Cells. G3:Genes/Genomes/Genetics (2019).

  2. H. Ishii et al., miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity. Nat Commun 9, (2018).
  3. E. Shifrut et al., Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell 175, (2018).
  4. J. Udeochu et al., Exosome release promotes inflammatory resolution in activated and aged microglia. bioRxiv, (2018).
  5. Z. Yang et al., Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors. Methods Mol Biol 1707, (2018).
  6. T. Roth et al., Reprogramming human T cell function and specificity with non-viral genome targeting. Nature (2018)
  7. G. Smith et al., IL-2Rβ abundance differentially tunes IL-2 signaling dynamics in CD4+ and CD8+ T cells. Sci Signaling 10, (2017).
  8. Z Yang et al. Regulation of B cell fate by chronic activity of the IgE B cell receptor. eLife (2016).

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Beschreibung
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Preis zzgl. MwSt.
04-165-5ug
 5ug