Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 

Canvax Biotech


Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research.

Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014.

This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history.

 
> Canvax Biotech > Produkte für die Forschung > Molekularbiologische Werkzeuge > Transfection reagents > Universal transfection reagents
Universal transfection reagents

Universal transfection reagents

Transfection is the process of introducing exogenous genetic material in the form of DNA or RNA into eukaryotic cells. The entry of this genetic material into the cell is allowed by the transient opening of pores in the membranes, it is then possible to introduce a plasmid, a small interfering RNA or other. Cells manipulated to accept a foreign nucleic acid are called competent cells. Transfection is not to be confused with transduction, which uses a different route of entry into the cell (through the use of a viral vector).

There are different methods of transfection, which are different chemical and/or physical treatments that will make the plasma membrane more permeable. The main methods used are :
  • Precipitation with calcium phosphate: the DNA will adhere to the membrane and enter the cell.
  • Lipofection, which will allow the DNA to be associated with cationic lipids, will create liposomes that will enter the cell by endocytosis.
  • Electroporation, which uses an electrical pulse to depolarize the membrane and open the pores.

 

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