Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 

Canvax Biotech


Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research.

Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014.

This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history.

 
> Canvax Biotech > Produkte für die Forschung > Molekularbiologische Werkzeuge > PCR products > Quantitative PCR - qPCR > qPCR mix > Mix for probes
Mix for probes

Mix for probes

Real-time quantitative PCR based on fluorescent probes relies on the specific detection of a sequence of a desired PCR product. Unlike methods based on SYBR green that detect all double-stranded DNAs, quantitative PCR using probes uses a fluorescently labeled specific probe, resulting in increased specificity and sensitivity. In addition, a variety of fluorescent dyes are available so that several primers can be used to simultaneously amplify many sequences.

Quantitative PCR using fluorescent probes is therefore ideal for high-throughput studies. The ready-to-use mixes contain all the necessary components for qPCR, simply add fluorescent detection probes, primers and DNA to be amplified.

Many fluorescent sources based on primers and probes for qPCR have been designed, including:
  • Hydrolysis Probes (TaqMan)
  • Molecular markers
  • Dual Hybridization Probes (FRET)
  • Eclipse Probes
  • Amplifluor assays

Here's how to select the appropriate ROX dye for the qPCR device you are using:

Universal All instruments
Without ROX Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROX Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROX Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus

High ROX
High ROX
Low ROX
Low ROX