TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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![G-418 (geneticin) for cell culture](https://www.clinisciences.com/de/upload/thumbs/bandeau3-mp5rq6aa-SAAy7gcR-786029.png)
G-418 (geneticin) for cell culture
The antibiotic G-418, also known as geneticin, is a selective antibiotic of the aminoglycoside family. G-418, produced by the bacterium Micromonospora rhodorangea, has a similar structure to gentamicin B1 and has the same mode of action on ribosomes. The classically used form of gentamicin is its disulfate.
G-418 has the particularity of inhibiting protein synthesis not only on bacteria but also on eukaryotic cells on which it has a cytotoxic effect. G-418 is said to be a selective antibiotic because it is possible to confer resistance to this antibiotic through the neo gene (neomycin resistance gene) of the Tn5 transposon. The genetically modified cells then carry the geneticin resistance gene. Selection in mammalian cells is usually achieved within 3-7 days with concentrations ranging from 400 to 1000 µg/ml.
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