TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Kits for the preparation of Illumina libraries - Difficult samples
The first step of a Next Generation Sequencing (NGS) is the preparation of a DNA bank. And for this NGS sequencing to be successful, the DNA bank must be of high quality. However, not all samples will yield good quality DNA for a good DNA bank. We then talk about difficult samples. These include FFPE samples, cell-free DNA samples or very small samples (also called single cells). Although these samples deliver DNA of poor quality and/or in very small quantities, their analysis gives results that are not possible with other samples. Examples include circulating tumour DNA or tumour biopsies. When starting with DNA in limited quantities or of variable quality, the bank preparation method must be precise and efficient, avoiding purification steps that reduce sample recovery.
