AP Chromogens for IHC

AP Chromogens for IHC



We offer a wide choice of specific chromogens for immunohistochemistry (IHC), for use with the 2 enzymes most used in this technique: horseradish peroxidase (HRP for Horseradish Peroxidase) and alkaline phosphatase (AP). Chromogens are chemical compounds used in IHC labeling procedures that produce a colored product that can be visualized by light microscopy. The choice of chromogen to use is directly related to the enzyme used in the test. Indeed, the enzyme will catalyze the conversion of the chromogenic substrate to produce a colored precipitate. Each enzyme can catalyze the conversion of specific substrates. Here are some examples of chromogens for HRP :

HRP:
  • Aminoethyl carbazole (AEC)
  • 3,3'-diaminobenzidine (DAB)
  • 3,3'5,5'-tetramethylbenzidine (TMB)
  • Emerald
AP:

Name
Color
Mounting
Purple blue
Aqueous
Red
Organic
Red
Aqueous