Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie
 
µ-Slide I Luer 3D

µ-Slide I Luer 3D

A µ-Slide with one channel and three wells for culturing cells on a 3D gel matrix with defined flow

  • Create a perfusable cell monolayer on a gel matrix for various flow and shear stress applications
  • Establish and image endothelial barriers under in vivo-like conditions—no artificial filters or membranes
  • Easy handling: samples are placed into the open wells; perfusion is applied after closing the wells

Applications

  • Culture of adherent cells on a 3D gel matrix under flow conditions
  • Transendothelial migration studies under flow conditions
  • Establishment and microscopic read-out of endothelial barrier assays (without any artificial membrane)
  • Simulation of blood vessels with endothelial cell culture on a soft substrate
  • Long-term or short-term cell culture perfusion experiments with defined shear stress and optimal nutrient supply into 3D structures
  • Apical-basal cell polarity assays
  • Cell barrier model assays with apical-basal gradients
  • Cell boundary assays without filters or porous membranes
  • Rolling and adhesion assays using leukocytes
  • Inflammation studies
  • Transport studies

Technical Features

  • Can create low or high shear stress in the 0.6 mm channel
  • Initial open well format allows for easy sample preparation, then is simply closed with a coverslip for the application of flow
  • The well design corrects meniscus formation and allows for superb cell visualization in one focal plane
  • Can be used with all ECM hydrogels
    (e.g., Collagen Type I, Matrigel, Fibrinogen)
  • Contains Luer adapters for easy tube and pump connection (e.g., to the ibidi Pump System)
  • ibidi Polymer Coverslip on top and bottom for high-resolution light microscopy
  • Partially compatible with upright microscopy
  • Defined shear stress levels

 

 

 

 

 

 

 The Principle of the µ-Slide I Luer 3D

The μ-Slide I Luer 3D has three wells with one channel on top for culturing cells on a 3D gel matrix with defined flow. Each well can be filled with a gel, on which cells can be cultivated and microscopically investigated. The channel can be connected to a pump (e.g., to the ibidi Pump System) for the application of defined shear stress.

Using this method, an in vivo-like functional monolayer (e.g., an endothelial barrier) can be established on the gel matrix without any artificial filters or membranes involved. The μ-Slide I Luer 3D is especially designed for the simulation of blood vessels using endothelial cells.

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